Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22002] to PDX1 - BSA and Azide free
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDX1 antibody [EPR22002] - BSA and Azide free
    See all PDX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22002] to PDX1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Human and rat species are recommended based on IHC result. This antibody is unsuitable to be used in western blot on human and rat samples.

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab234633 is the carrier-free version of ab219207.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)
    Western blot - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)
    All lanes : Anti-PDX1 antibody at 1/1000 dilution

    Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 31 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207 & ab134150).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining on human pancreatic islet and pancreatic acini (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)
    Immunocytochemistry/ Immunofluorescence - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling PDX1 with ab219207 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining in Beta-TC-6 cells.

    Negative control: NIH/3T3 (PMID: 25271154).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207).

  • Immunoprecipitation - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)
    Immunoprecipitation - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)

    PFX1 was immunoprecipitated from 0.35 mg of Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate with ab219207 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219207 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Beta-TC-6 whole cell lysate 10 µg (Input). 
    Lane 2: ab219207 IP in Beta-TC-6 whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219207 in Beta-TC-6 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 12488243).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining in rat pancreatic islet (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling PDX1 with ab219207 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Nuclear and weak cytoplasmic staining in mouse pancreatic islet (PMID: 1847781, PMID: 22688334) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219207).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)
    Anti-PDX1 antibody [EPR22002] - BSA and Azide free (ab234633)

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