Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17413] to CSDE1/NRU - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free
    See all CSDE1/NRU primary antibodies

  • Description

    Rabbit monoclonal [EPR17413] to CSDE1/NRU - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251319 is the carrier-free version of ab200663 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251319 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as CSDE1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    All lanes : Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/2000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa


    Exposure time: 3 minutes


    This data was developed using ab200663, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution + MCF-7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa


    Exposure time: 3 minutes


    This data was developed using ab200663, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution + Human fetal brain tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa


    Exposure time: 3 minutes


    This data was developed using ab200663, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    All lanes : Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa


    Exposure time: 1 minute


    This data was developed using ab200663, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    All lanes : Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/2000 dilution

    Lane 1 : HeLa cytosolic fraction
    Lane 2 : HeLa membrane (mitochondrial) fraction
    Lane 3 : HeLa nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 89 kDa
    Observed band size: 89 kDa


    Exposure time: 3 minutes


    This data was developed using ab200663, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The HeLa cell fractions lysate set is ab168542 from Abcam.

  • Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    This data was developed using ab200663, the same antibody clone in a different buffer formulation.CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab200663 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    This data was developed using ab200663, the same antibody clone in a different buffer formulation.CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/1000 dilution (Panel A) or ab201688 at 1/1000 dilution (Panel B). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution. Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab200663 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 min (Panel A); 10 seconds (Panel B).
  • Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)
    Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (ab251319)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.