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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Human ERGIC2 (PTX1) knockout HeLa cell lysate (ab258862)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human ERGIC2 (PTX1) knockout HeLa cell lysate (ab258862)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human ERGIC2 (PTX1) knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 0 bp insertion in exon 2 and 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261373 - Human ERGIC2 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10

Target

  • Function

    Possible role in transport between endoplasmic reticulum and Golgi.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the ERGIC family.
  • Cellular localization

    Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus > cis-Golgi network membrane. Endoplasmic reticulum membrane. Cytoplasm. Nucleus. Cycles between the endoplasmic reticulum and the Golgi. PubMed:11445006 states that it localizes to the nucleus; PubMed:17980171 shows a partial localization in the nucleus.
  • Target information above from: UniProt accession Q96RQ1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • cd002
    • CD14 protein
    • CDA14
    • Endoplasmic reticulum Golgi intermediate compartment protein 2
    • Endoplasmic reticulum-Golgi intermediate compartment protein 2
    • ERGI2_HUMAN
    • ERGIC 2
    • ERGIC and golgi 2
    • ergic2
    • Erv41
    • MGC111152
    • PTX1
    • PTX1 protein
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261373 - Human ERGIC2 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10

Images

  • Sanger Sequencing - Human ERGIC2 knockout HeLa cell lysate (ab258862)
    Sanger Sequencing - Human ERGIC2 knockout HeLa cell lysate (ab258862)

    Allele-1: 1 bp deletion in exon 2

     

  • Sanger Sequencing - Human ERGIC2 knockout HeLa cell lysate (ab258862)
    Sanger Sequencing - Human ERGIC2 knockout HeLa cell lysate (ab258862)

    Allele-2: 0 bp insertion in exon 2

     

  • Sanger Sequencing - Human ERGIC2 knockout HeLa cell lysate (ab258862)
    Sanger Sequencing - Human ERGIC2 knockout HeLa cell lysate (ab258862)

    Allele-3: Insertion of the selection cassette in exon 2

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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