Anti-Hsp22/HSPB8 antibody (ab96837)
Key features and details
- Rabbit polyclonal to Hsp22/HSPB8
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp22/HSPB8 antibody
See all Hsp22/HSPB8 primary antibodies -
Description
Rabbit polyclonal to Hsp22/HSPB8 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Hsp22/HSPB8 aa 134-196. The exact sequence is proprietary.
Database link: Q9UJY1 -
Positive control
- WB: HEK-293T, A431 cell lysate. ICC/IF: HeLa cells. IHC-P: Human lung SCC. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skeletal muscle.
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General notes
This product was previously labelled as Hsp22
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hsp22/HSPB8 antibody (ab96837)All lanes : Anti-Hsp22/HSPB8 antibody (ab96837) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody (ab96837)Paraffin embedded human lung SCC tissue stained for HSP22 using ab96837 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp22/HSPB8 antibody (ab96837)Immunofluorescence analysis of Hsp22/HSPB8 expression in PFA-fixed HeLa cells, using ab96837 at 1/200 dilution. Lower image was merged with DNA probe.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody (ab96837)IHC image of Hsp22/HSPB8 staining in Human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96837, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
