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Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9714] to Hsp22/HSPB8
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Hsp22/HSPB8 antibody [EPR9714]
    See all Hsp22/HSPB8 primary antibodies

  • Description

    Rabbit monoclonal [EPR9714] to Hsp22/HSPB8

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Hsp22/HSPB8. The immunogen used for this product shares 64% homology with Hsp27. Cross-reactivity with this protein has not been confirmed experimentally.

  • Positive control

    • Fetal heart and fetal muscle lysates; Human skeletal muscle tissue; A673 cells

  • General notes

     This product was previously labelled as Hsp22

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
    Western blot - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
    All lanes : Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552) at 1/1000 dilution

    Lane 1 : Fetal heart lysate
    Lane 2 : Fetal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Hsp22/HSPB8 with ab151552 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

    Immunofluorescent analysis of A673 cells labeling Hsp22/HSPB8 with ab151552 at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

    Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151552 showing -ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

    Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151552 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

    Immunohistochemical analysis of paraffin embedded Human Gastric carcinoma tissue using ab151552 showing -ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

    Immunohistochemical analysis of paraffin embedded Mouse brain tissue using ab151552 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

    Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151552 showing -ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)
    Anti-Hsp22/HSPB8 antibody [EPR9714] (ab151552)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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