Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13349] to DNAJC7 - N-terminal
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-DNAJC7 antibody [EPR13349] - N-terminal
    See all DNAJC7 primary antibodies

  • Description

    Rabbit monoclonal [EPR13349] to DNAJC7 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human DNAJC7 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q99615

  • Positive control

    • HeLa, HepG2, 293T and Jurkat cell lysates; Human breast tissue and colonic adenocarcinoma tissue; HepG2 and HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13349

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
    Western blot - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
    All lanes : Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
    Immunocytochemistry/ Immunofluorescence - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)

    Immunofluorescent analysis of HepG2 cells labeling DNAJC7 with ab179830 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)

    Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling DNAJC7 with ab179830 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
    Immunoprecipitation - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)

    Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab179830 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Flow Cytometry - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
    Flow Cytometry - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)

    Flow cytometric analysis of permeabilized HeLa cells labeling DNAJC7 with ab179830 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DNAJC7 with ab179830 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)
    Anti-DNAJC7 antibody [EPR13349] - N-terminal (ab179830)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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