Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325)
![Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325)](https://www.abcam.com/ps/products/241/ab241325/Images/ab241325-336115-anti-erbb-2-antibody-cpt-r322-27-12-western-blot.jpg "Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CPT-R32.2-27-12] to ErbB2 / HER2
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: Human
Overview
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Product name
Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12]
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit monoclonal [CPT-R32.2-27-12] to ErbB2 / HER2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SK-BR-3 whole cell lysate. ICC/IF: SK-BR-3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CPT-R32.2-27-12 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325)All lanes : Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325) at 1/1000 dilution
Lane 1 : SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 137 kDa
Observed band size: 150-250 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsNegative control-MCF7. MCF7 belongs to Luminal A subtype which is HER2 negative (PMID: 20697531).
HER2 has 6 potential glycosylation sites, which might lead to the molecular mass greater than the predicted size. The expression profile in SK-BR-3 cells is consistent with literature (PMID: 24425042). The multiple bands likely represent degraded HER2 or different isoforms.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325)](https://www.abcam.com/ps/products/241/ab241325/Images/ab241325-336117-anti-erbb-2-antibody-cpt-r322-27-12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325)Immunofluorescent analysis of 100% methanol fixed SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labeling ErbB 2 with ab241325 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membranous staining in SK-BR-3 cells.
Negative control: MCF7 (Human breast adenocarcinoma cell line) (PMID:20697531). Negative staining was obtained from 4% PFA fixation.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
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![ELISA - Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325)](https://www.abcam.com/ps/products/241/ab241325/Images/ab241325-336118-anti-erbb-2-antibody-cpt-r322-27-12-elisa.jpg)
ELISA - Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325)ELISA - Anti-ErbB 2 antibody [CPT-R32.2-27-12] (ab241325) used at 1000 ng/mL.
50 ng CPTAC-36d (1000 ng/mL, 50 μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000 ng/mL) of ab241325 (50 μL) were incubated with CPTAC-36d in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50 μL, 1/2500) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
Primary antibody concentration range: 0.01 - 1.0 µg/mL.
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![Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325)](https://www.abcam.com/ps/products/241/ab241325/Images/ab241325-5-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 antibody [CPT-R32.2-27-12] (ab241325)
