Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [mG2Na-s] (ab15672)
![Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [mG2Na-s] (ab15672)](https://www.abcam.com/ps/products/15/ab15672/Images/ab15672-23318-anti-metabotropic-glutamate-receptor-2-antibody-mg2na-s-western-blot.jpg "Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [mG2Na-s] (ab15672)")
Key features and details
- Mouse monoclonal [mG2Na-s] to Metabotropic Glutamate Receptor 2/MGLUR2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [mG2Na-s]
See all Metabotropic Glutamate Receptor 2/MGLUR2 primary antibodies -
Description
Mouse monoclonal [mG2Na-s] to Metabotropic Glutamate Receptor 2/MGLUR2 -
Host species
Mouse -
Specificity
This antibody recognizes the metabotrophic glutamate receptor 2, but not metabotrophic glutamate receptor 3, in rat and mouse. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Fusion protein corresponding to Metabotropic Glutamate Receptor 2/MGLUR2. Fusion protein contains a 47 amino acid sequence of the N terminal of the Metabotrophic Glutamate Receptor 2
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Positive control
- WB: Rat brain lysate. IHC: Mouse cortical neurons.
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General notes
This product was previously labelled as Metabotropic Glutamate Receptor 2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 0.02% Potassium chloride, 0.04% Potassium phosphate, 0.81% Sodium chloride, 0.16% Sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
mG2Na-s -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [mG2Na-s] (ab15672)Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [mG2Na-s] (ab15672) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesThe band observed at 99 kDa could potentially be a cleaved form of Metabotropic Glutamate Receptor 2/MGLUR2 due to the presence of an 18 amino acid signal peptide. Metabotropic Glutamate Receptor 2/MGLUR2 also contains a number of potential glycosylation sites (SwissProt) which may explain the fuzzy banding pattern observed.
