Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [WM15] to CD13 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CD13 antibody [WM15] - BSA and Azide free
    See all CD13 primary antibodies

  • Description

    Mouse monoclonal [WM15] to CD13 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    The antibody recognizes an extracellular epitope.

  • Positive control

    • ICC: A375 and THP-1 cells. Flow Cyt: THP-1 cells and Human peripheral blood leukocytes.

  • General notes

    ab252261 is the PBS only format of ab7417.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
    Flow Cytometry - Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)

    This data was developed using the same antibody clone in a different buffer formulation (ab7417)

    Flow cytometry overlay histogram showing wild-type THP1 (green line) and ANPEP knockout THP1 cells (ab273759) stained with ab7417 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab7417) (1x106 in 100μl at 1 μg/ml) for 30 min at 4°C.

    The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.

    Isotype control antibody was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody (wild-type THP1 cells - black line; ANPEP knockout THP1 cells ab273759 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Immunocytochemistry - Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
    Immunocytochemistry - Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
    This data was developed using the same antibody clone in a different buffer formulation (ab7417). ab7417 staining CD13 in wild-type THP-1 cells (top panel) and ANPEP knockout THP-1 cells (bottom panel) (ab273759). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7417 at 2.5μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Flow Cytometry - Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
    Flow Cytometry - Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab7417).

    ab7417 staining CD13 on the surface of human peripheral blood leukocytes in Flow Cytometry.

  • Immunocytochemistry - Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
    Immunocytochemistry - Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab7417).

    ab7417 staining CD13 in A375 (Human epithelial cell line from skin malignant melanoma) cells.

    The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab7417 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also produced a positive signal in A375 when fixed with 4% formaldehyde (10min).

  • Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
    Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)

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