Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] - BSA and Azide free (ab238432)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2 - BSA and Azide free
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] - BSA and Azide free
See all Metabotropic Glutamate Receptor 2/MGLUR2 primary antibodies -
Description
Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat brain lysates. IHC-P: Human fetal brain tissue. Flow Cyt: SH-SY-SY cells.
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General notes
ab238432 is the carrier-free version of ab150387. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab238432 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Metabotropic Glutamate Receptor 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8975 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 95 kDa
Observed band size: 95 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 180 s
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Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Metabotropic Glutamate Receptor 2/MGLUR2 with unpurified ab150387 at 1/70 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150387).
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Immunohistochemical analysis of Human fetal brain tissue labelling Metabotropic Glutamate Receptor 2/MGLUR2 with ab150387 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150387).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab150387 showing -ve staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150387).Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150387 showing -ve staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150387).Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab150387 showing +ve staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150387).Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150387 showing -ve staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150387).Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Fetal kidney tissue using ab150387 showing -ve staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150387).Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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