SOCS4 overexpression 293T lysate (whole cell) (ab94295)
Overview
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Product name
SOCS4 overexpression 293T lysate (whole cell)
See all SOCS4 lysates -
General notes
ab94295 is a 293T cell transfected lysate in which Human SOCS4 has been transiently over-expressed using a pCMV-SOCS4 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Pathway: Protein modification; protein ubiquitination. Similarity: Contains 1 SH2 domain. Contains 1 SOCS box domain. Domain: The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
Images
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SDS-PAGE - SOCS4 overexpression 293T lysate (whole cell) (ab94295)ab94295 at 15µg/lane on an SDS-PAGE gel.
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Western blot - SOCS4 overexpression 293T lysate (whole cell) (ab94295)All lanes : Anti-SOCS4 antibody (ab57337) at 1/500 dilution
Lane 1 :SOCS4 overexpression 293T lysate (whole cell) (ab94295)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution