Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PLA2R antibody [CL0474] (ab188028)

Key features and details

  • Mouse monoclonal [CL0474] to PLA2R
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PLA2R antibody [CL0474]
    See all PLA2R primary antibodies

  • Description

    Mouse monoclonal [CL0474] to PLA2R

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human PLA2R aa 350-550. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q13018

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    Binds to an epitope located within the peptide sequence KIPVSFEWSN as determined by overlapping synthetic peptides.

  • Positive control

    • IHC-P: Human kidney with membranous nephropathy; WB: Human kidney tissue lysate; ICC: HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [CL0474] (ab188028)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [CL0474] (ab188028)

    Immunohistochemical staining (using ab188028) of human kidney with membranous nephropathy shows strong membranous immunoreactivity in renal glomeruli.

  • Immunocytochemistry - Anti-PLA2R antibody [CL0474] (ab188028)
    Immunocytochemistry - Anti-PLA2R antibody [CL0474] (ab188028)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PLA2R with ab188028 at 10 µg/mL, showing specific staining in the cytosol and plasma membrane in green. Nuclear probes are visualized in blue.

     

  • Western blot - Anti-PLA2R antibody [CL0474] (ab188028)
    Western blot - Anti-PLA2R antibody [CL0474] (ab188028)
    Anti-PLA2R antibody [CL0474] (ab188028) at 1 µg/ml + Human kidney tissue lysate

    Predicted band size: 169 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [CL0474] (ab188028)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [CL0474] (ab188028)

    Immunohistochemical staining (using ab188028) of human kidney shows only low background positivity.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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