RNase Activity Assay Kit (Fluorometric) (ab273299)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Other biological fluids
- Sensitivity: 0.4 pg/ml
Overview
-
Product name
RNase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Other biological fluids -
Assay type
Enzyme activity (quantitative) -
Sensitivity
0.4 pg/ml -
Assay duration
Multiple steps standard assay -
Product overview
RNase Activity Assay Kit (Fluorometric) (ab273299) enables researchers to measure RNase activity in buffers, reagents, and other components as well as quantitatively evaluate RNAse activity of recombinant enzymes in real time.
The assay uses a highly sensitive, specific probe that releases a fluorescent product in the presence of active RNAse. The limit of detection is 0.4 pg RNase/well and limit of quantification is 1.2 pg RNase/well.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 10X RNase Buffer 1 x 1ml Fluorescence Standard 1 x 100µl Half-area Plate 1 unit Molecular Biology Grade Water 1 x 25ml RNA Probe (Lyophilized) 1 vial RNase Positive Control 1 x 250µl
Images
-
Fluorescence Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
-
Sample Kinetic CurvesSample kinetic curve obtained from Negative Control (Blank), bacterial culture, RNase contaminated BSA and purified RNase.
