All lanes : Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] (ab85320) at 1/500 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CSNK1D knockout HeLa cell lysate
Lane 3 : K-562 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 47 kDa
Observed band size: 47 kDa

Lanes 1-3: Merged signal (red and green). Green - ab85320 observed at 47 kDa. Red - loading control, ab181602 observed at 37 kDa.

ab85320 Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] was shown to specifically react with Casein Kinase 1 delta/CSNK1D in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264667 (knockout cell lysate ab258383) was used. Wild-type and Casein Kinase 1 delta/CSNK1D knockout samples were subjected to SDS-PAGE. ab85320 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

 

Allele-1: 1 bp deletion in exon 1.

 

Allele-2: 11 bp insertion in exon 1.