Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DLC-A antibody [EPR11240] (ab157468)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11240] to DLC-A
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-DLC-A antibody [EPR11240]
    See all DLC-A primary antibodies

  • Description

    Rabbit monoclonal [EPR11240] to DLC-A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human DLC-A.
    Database link: Q9Y6G9

  • Positive control

    • K562, HeLa, fetal brain, fetal heart and Jurkat lysates; Human heart and pancreas tissues; K562 cells; Immunoprecipitation pellet from fetal brain lysate.

  • General notes

    Previously labelled as Cytoplasmic dynein 1 light intermediate chain 1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11240

  • Isotype

    IgG

Images

  • Western blot - Anti-DLC-A antibody [EPR11240] (ab157468)
    Western blot - Anti-DLC-A antibody [EPR11240] (ab157468)
    All lanes : Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Fetal brain lysate
    Lane 4 : Fetal heart lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] (ab157468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] (ab157468)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling DLC-A with ab157468 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] (ab157468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] (ab157468)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DLC-A with ab157468 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DLC-A antibody [EPR11240] (ab157468)
    Immunocytochemistry/ Immunofluorescence - Anti-DLC-A antibody [EPR11240] (ab157468)

    Immunofluorescent analysis of K562 cells labeling DLC-A with ab157468 at 1/100 dilution.

  • Western blot - Anti-DLC-A antibody [EPR11240] (ab157468)
    Western blot - Anti-DLC-A antibody [EPR11240] (ab157468)
    Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution + immunoprecipitation pellet from fetal brain lysate

    Secondary
    HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.

    Predicted band size: 57 kDa

  • Anti-DLC-A antibody [EPR11240] (ab157468)
    Anti-DLC-A antibody [EPR11240] (ab157468)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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