Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E398L] to Lamin B Receptor/LBR - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free
    See all Lamin B Receptor/LBR primary antibodies

  • Description

    Rabbit monoclonal [E398L] to Lamin B Receptor/LBR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IF: HeLa cells. WB: Jurkat cell lysate. HeLa: HEK-293 cell lysate. Flow Cyt: HeLa cells.

  • General notes

    ab222391 is the carrier-free version of ab32535 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab222391 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Lamin B Receptor

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)
    Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)
    All lanes : Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535) at 1/500 dilution

    Lane 1 : Wild-type HEK-293 cell lysate
    Lane 2 : Human LBR (Lamin B Receptor) knockout HEK-293T cell lysate (ab257503)

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 71 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab32535).

    Lanes 1 - 2: Merged signal (red and green). Green - ab32535 observed at 58 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab32535 was shown to react with Lamin B Receptor/LBR in wild-type HEK-293 cells in western blot. Loss of signal was observed when LBR knockout cell lysate ab257503 was used. Wild-type and LBR knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab32535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)

    Ab32535, at a 1/500 dilution, staining Lamin B Receptor/LBR in HeLa cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32535).

  • Flow Cytometry - Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)
    Flow Cytometry - Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Lamin B Receptor/LBR with unpurified ab32535 at 1/20 dilutionc(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32535).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)
    IHC image of ab32535 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32535, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32535).

  • Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)
    Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)

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