Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E398L] to Lamin B Receptor/LBR
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Lamin B Receptor/LBR antibody [E398L]
    See all Lamin B Receptor/LBR primary antibodies

  • Description

    Rabbit monoclonal [E398L] to Lamin B Receptor/LBR

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Lamin B Receptor/LBR. The exact sequence is proprietary.

  • Positive control

    • IF: HeLa cells. WB: HEK-293 and Jurkat whole cell lysate (ab7899). Flow Cyt: HeLa cells. IHC-P: Human breast carcinoma tissue.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Lamin B Receptor

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
    Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
    All lanes : Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535) at 1/500 dilution

    Lane 1 : Wild-type HEK-293 cell lysate
    Lane 2 : Human LBR (Lamin B Receptor) knockout HEK-293T cell lysate (ab257503)

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 71 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab32535 observed at 58 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab32535 was shown to react with Lamin B Receptor/LBR in wild-type HEK-293 cells in western blot. Loss of signal was observed when LBR knockout lysate ab257503 was used. Wild-type and LBR knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab32535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)

    Ab32535, at a 1/500 dilution, staining Lamin B Receptor/LBR in HeLa cells by Immunofluorescence.

  • Flow Cytometry - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
    Flow Cytometry - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Lamin B Receptor/LBR with unpurified ab32535 at 1/20 dilutionc(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
    IHC image of ab32535 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32535, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
    Western blot - Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
    Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535) at 1/500 dilution + Jurkat cell lysate

    Predicted band size: 71 kDa
    Observed band size: 67 kDa why is the actual band size different from the predicted?

  • Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
    Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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