Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GNA13 antibody [EPR5436] (ab128900)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5436] to GNA13
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GNA13 antibody [EPR5436]
    See all GNA13 primary antibodies

  • Description

    Rabbit monoclonal [EPR5436] to GNA13

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GNA13 aa 300 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB: Y79, 293T, HeLa and HepG2 cell lysates. IHC-P: Human bladder carcinoma and kidney tissues

  • General notes

     This product was previously labelled as G protein alpha 13

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 6.10 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5436

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GNA13 antibody [EPR5436] (ab128900)
    Western blot - Anti-GNA13 antibody [EPR5436] (ab128900)
    All lanes : Anti-GNA13 antibody [EPR5436] (ab128900) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : GNA13 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab128900 observed at 45 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab128900 was shown to react with GNA13 in wild-type HeLa cells in Western blot with loss of signal observed in GNA13 knockout cell line ab264846 (GNA13 knockout cell lysate ab257451). Wild-type HeLa and GNA13 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab128900 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (ab128900)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (ab128900)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling GNA13 with Purified ab128900 at 1:1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-GNA13 antibody [EPR5436] (ab128900)
    Western blot - Anti-GNA13 antibody [EPR5436] (ab128900)
    All lanes : Anti-GNA13 antibody [EPR5436] (ab128900) at 1/1000 dilution (unpurified)

    Lane 1 : Y79 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 44 kDa

  • Western blot - Anti-GNA13 antibody [EPR5436] (ab128900)
    Western blot - Anti-GNA13 antibody [EPR5436] (ab128900)
    All lanes : Anti-GNA13 antibody [EPR5436] (ab128900) at 1/5000 dilution (purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-GNA13 antibody [EPR5436] (ab128900)
    Western blot - Anti-GNA13 antibody [EPR5436] (ab128900)
    All lanes : Anti-GNA13 antibody [EPR5436] (ab128900) at 1/5000 dilution (unpurified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 30 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (ab128900)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (ab128900)

    Unpurified ab128900, at a 1/250 dilution, staining GNA13 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (ab128900)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (ab128900)

    Unpurified ab128900, at a 1/250 dilution, staining GNA13 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (ab128900)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (ab128900)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling GNA13 with Purified ab128900 at 1:1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (ab128900)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] (ab128900)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling GNA13 with Purified ab128900 at 1:1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • OI-RD Scanning - Anti-GNA13 antibody [EPR5436] (ab128900)
    OI-RD Scanning - Anti-GNA13 antibody [EPR5436] (ab128900)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-GNA13 antibody [EPR5436] (ab128900)
    Anti-GNA13 antibody [EPR5436] (ab128900)

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