Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5436] to GNA13 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GNA13 antibody [EPR5436] - BSA and Azide free
    See all GNA13 primary antibodies

  • Description

    Rabbit monoclonal [EPR5436] to GNA13 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human bladder carcinoma and kidney tissue; Rat stomach tissue; Mouse stomach tissue. WB: HeLa, HepG2 and LNCaP cell lysates.

  • General notes

    ab232478 is the carrier-free version of ab128900 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232478 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as G protein alpha 13

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)
    Western blot - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)
    All lanes : Anti-GNA13 antibody [EPR5436] (ab128900) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : GNA13 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab128900).

    Lanes 1 - 2: Merged signal (red and green). Green - ab128900 observed at 45 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab128900 was shown to react with GNA13 in wild-type HeLa cells in Western blot with loss of signal observed in GNA13 knockout cell line ab264846 (GNA13 knockout cell lysate ab257451). Wild-type HeLa and GNA13 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab128900 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling GNA13 with Purified ab128900 at 1:1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128900).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)

    Unpurified ab128900, at a 1/250 dilution, staining GNA13 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128900).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)

    Unpurified ab128900, at a 1/250 dilution, staining GNA13 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128900).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling GNA13 with Purified ab128900 at 1:1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128900).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling GNA13 with Purified ab128900 at 1:1000 dilution (1.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128900).

  • OI-RD Scanning - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)
    OI-RD Scanning - Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128900).

  • Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)
    Anti-GNA13 antibody [EPR5436] - BSA and Azide free (ab232478)

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