Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13657] to Mad2L2/REV7 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free
    See all Mad2L2/REV7 primary antibodies

  • Description

    Rabbit monoclonal [EPR13657] to Mad2L2/REV7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human ovarian carcinoma tissue.

  • General notes

    ab232620 is the carrier-free version of ab180579 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232620 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Mad2L2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)
    Immunocytochemistry/ Immunofluorescence - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Mad2L2/REV7 with ab129199 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180579).

  • Immunoprecipitation - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)
    Immunoprecipitation - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)

    Immunoprecipitation of Hela cells labeling Mad2L2/REV7 using ab180579 at 1/40 dilution (lane 1) or negative control (lane 2) followed by anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.  

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180579).

  • Flow Cytometry - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)
    Flow Cytometry - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)

    Flow cytometric analysis of paraformadehyde fixed K562 cells labeling Mad2L2/REV7 with ab180579 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/50.  

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180579).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)

    Immunohistochemical staining of paraffin-embedded human ovarian carcinoma tissue labeling Mad2L2/REV7 using ab180579 at a 1/50 dilution and ImmunoHistoprobe HRP Polymer for Rabbit IgG.  Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180579).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)
    Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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