Human ErbB3 ELISA Kit (HER3) (ab275101)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 27.6 pg/ml
- Range: 109.4 pg/ml - 7000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ErbB3 ELISA Kit (HER3)
See all ErbB3 / HER3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.8% Inter-assay Sample n Mean SD CV% Serum 3 4.8% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
27.6 pg/ml -
Range
109.4 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 83 82% - 83% Saliva 106 104% - 107% Urine 90 83% - 93% Serum 94 92% - 96% Cell Lysate 97 90% - 106% Hep Plasma 114 112% - 117% EDTA Plasma 91 88% - 94% Cit plasma 92 88% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human ErbB 3 ELISA kit (ab275101) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ErbB 3 protein in serum, plasma, cell culture supernatant, urine, saliva and cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ErbB 3 with 27.6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Receptor tyrosine-protein kinase ErbB 3, also known human epidermal growth factor receptor 3 (HER3), is encoded by the ERBB3 gene in which is a member of the EGFR family. ErbB 3 is a type I transmembrane glycoprotein that binds to neuregulins NRG1 and NRG2, but lacks a working kinase domain. ErbB 3 is capable of heterodimerization of its fellow ErbB family members, most notably with ErbB 2 for signaling. Moreover, ErbB 3 is vital in the development of Schwann cells, neural crest differentiation, sympathetic nervous system, and heart values. Multiple studies have shown ErbB 3 to play roles in cellular growth, proliferation, differentiation, promotion and metastasis of cancer, and chemotherapeutic resistance.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ErbB 3 (HER3) Capture Antibody 1 x 600µl 10X Human ErbB 3 (HER3) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human ErbB 3 (HER3) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds and is activated by neuregulins and NTAK. -
Tissue specificity
Epithelial tissues and brain. -
Involvement in disease
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Developmental stage
Overexpressed in a subset of human mammary tumors. -
Domain
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
Post-translational
modificationsLigand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- c erbB 3
- c erbB3
- Erb b2 receptor tyrosine kinase 3
see all -
Database links
- Entrez Gene: 2065 Human
- Omim: 190151 Human
- SwissProt: P21860 Human
- Unigene: 118681 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ErbB 3 standard curve in Sample Diluent NS.The ErbB 3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ErbB 3 in human serum, plasma and cell culture supernatant samples.The concentrations of ErbB 3 were measured in duplicates, interpolated from the ErbB 3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25% and MCF-7 supernatant neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ErbB 3 concentration was determined to be 4,877 pg/mL in serum, 3,874 pg/mL in plasma (citrate), 4,148 pg/mL in plasma (EDTA), 3,783 pg/mL in plasma (heparin), and 301.2 pg/mL in MCF-7 supernatant.
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Interpolated concentrations of native ErbB 3 in human MCF-7 based on an 800 µg/mL extract load.The concentrations of ErbB 3 were measured in duplicate and interpolated from the ErbB 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ErbB 3 concentration was determined to be 3,799 pg/mL in MCF-7 cell extract.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ErbB 3 concentration was determined to be 4561 pg/mL with a range of 3275 pg/mL – 6077 pg/mL.
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Sandwich ELISA - Human ErbB3 / HER3 ELISA Kit (HER3) (ab275101)To learn more about the advantages of recombinant antibodies see here.
