Anti-GNB2 antibody [EP3262Y] - BSA and Azide free (ab247673)
![Anti-GNB2 antibody [EP3262Y] - BSA and Azide free (ab247673)](https://www.abcam.com/ps/products/247/ab247673/Images/ab247673-393689-anti-gnb2-antibody-ep3262y-western-blot-human-knockout-lysate.jpg "Anti-GNB2 antibody [EP3262Y] - BSA and Azide free (ab247673)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3262Y] to GNB2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-GNB2 antibody [EP3262Y] - BSA and Azide free
See all GNB2 primary antibodies -
Description
Rabbit monoclonal [EP3262Y] to GNB2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, Jurkat and SH-SY5Y cell lysates.
-
General notes
Ab247673 is the carrier-free version of ab108504. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247673 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3262Y -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-GNB2 antibody [EP3262Y] - BSA and Azide free (ab247673)All lanes : Anti-GNB2 antibody [EP3262Y] (ab108504) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GNB2 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab108504).
Lanes 1-4: Merged signal (red and green). Green - ab108504 observed at 35 kDa. Red - loading control ab7291 observed at 50 kDa.
ab108504 Anti-GNB2 antibody [EP3262Y] was shown to specifically react with GNB2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266528 (knockout cell lysate ab257453) was used. Wild-type and GNB2 knockout samples were subjected to SDS-PAGE. ab108504 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Anti-GNB2 antibody [EP3262Y] - BSA and Azide free (ab247673)](https://www.abcam.com/ps/products/247/ab247673/Images/ab247673-2-benefits-of-recombinant-antibodies.png)
Anti-GNB2 antibody [EP3262Y] - BSA and Azide free (ab247673)
