Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)
![Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)](https://www.abcam.com/ps/products/76/ab76469/Images/ab76469-91900-anti-erbb-3-phospho-y1289-antibody-epr2325-immunofluorescence.gif "Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2325] to ErbB3 / HER3 (phospho Y1289)
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325]
See all ErbB3 / HER3 primary antibodies -
Description
Rabbit monoclonal [EPR2325] to ErbB3 / HER3 (phospho Y1289) -
Host species
Rabbit -
Specificity
ab76469 only detects ErbB 3 phosphorylated on Tyrosine 1289. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
corresponding to Human ErbB3/ HER3. A phospho-specific peptide corresponding to residues surrounding Tyrosine 1289 of Human ErbB3/ HER3
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Positive control
- SKBR3 cell lysate treated with Neuregulin. MCF7 cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2325 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)Immunofluorescent staining of MCF7 cells using ab76469 at 1/100 dilution.
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![Western blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)](https://www.abcam.com/ps/products/76/ab76469/Images/ab76469-91901-anti-erbb-3-phospho-y1289-antibody-epr2325-western-blot.jpg)
Western blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)All lanes : Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469) at 1/5000 dilution
Lane 1 : SKBR3 cell lysate untreated
Lane 2 : SKBR3 cell lysate treated with Neuregulin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
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![Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)](https://www.abcam.com/ps/products/76/ab76469/Images/ab76469-3-benefits-of-recombinant-antibodies.png)
Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)
