Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPNCIR124] (ab133443)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR124] to ErbB3 / HER3 (phospho Y1289)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPNCIR124]
See all ErbB3 / HER3 primary antibodies -
Description
Rabbit monoclonal [EPNCIR124] to ErbB3 / HER3 (phospho Y1289) -
Host species
Rabbit -
Specificity
ab133433 only detects ErbB3 / HER3 phosphorylated at Tyrosine 1289.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
corresponding to Mouse ErbB3/ HER3. Synthetic phosphopeptide corresponding to a region surrounding Tyrosine 1289 of Mouse ErbB3/ HER3.
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Positive control
- Lysate of MCF7 cells treated with neuregulin.
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institutes Center for Cancer Research and the lab of Terry Van Dyke. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPNCIR124 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPNCIR124] (ab133443) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : MCF7 cell lysate treated with neuregulin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/10000 dilution
Predicted band size: 148 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
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