Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ErbB3 / HER3 antibody [E186] (ab32121)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E186] to ErbB3 / HER3
Suitable for: WB
Knockout validated
Reacts with: Rat, Human

Product name

Anti-ErbB3 / HER3 antibody [E186]
See all ErbB3 / HER3 primary antibodies
Description

Rabbit monoclonal [E186] to ErbB3 / HER3
Host species

Rabbit
Specificity

ab32121 recognises ErbB3/HER3. WB samples: should avoid boiling
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human ErbB3/ HER3 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P21860
Positive control
- MCF-7 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

E186
Isotype

IgG
Research areas

Western blot - Anti-ErbB3 / HER3 antibody [E186] (ab32121)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ERBB3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: MCF7 whole cell lysate (20 µg)

Lanes 1 - 3: Merged signal (red and green). Green - ab32121 observed at 185 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab32121 was shown to specifically recognize ERBB3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when knock out samples were examined. Wild-type and ERBB3 knockout samples were subjected to SDS-PAGE. Ab32121 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-ErbB3 / HER3 antibody [E186] (ab32121)

Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/1000 dilution + MDA-MB-435S (human ductal carcinoma) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 149 kDa
Western blot - Anti-ErbB3 / HER3 antibody [E186] (ab32121)

All lanes : Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 2.685 µg/ml (purified)

Lane 1 : MDA-MB-435S (human mammary gland ductal carcinoma melanocyte) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 3 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 149 kDa

Exposure time: 37 seconds
Western blot - Anti-ErbB3 / HER3 antibody [E186] (ab32121)

Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/500 dilution (unpurified) + MCF-7 cell lysate

Predicted band size: 149 kDa
Observed band size: 185 kDa
why is the actual band size different from the predicted?
Anti-ErbB3 / HER3 antibody [E186] (ab32121)

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