Anti-ErbB3 / HER3 (phospho Y1222) antibody [EPR5807] (ab133445)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5807] to ErbB3 / HER3 (phospho Y1222)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-ErbB3 / HER3 (phospho Y1222) antibody [EPR5807]
See all ErbB3 / HER3 primary antibodies -
Description
Rabbit monoclonal [EPR5807] to ErbB3 / HER3 (phospho Y1222) -
Host species
Rabbit -
Specificity
ab133445 only detects ErbB3 / HER3 phosphorylated at Tyrosine 1222.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
corresponding to Human ErbB3/ HER3. Synthetic phosphopeptide corresponding to a region surrounding Tyrosine 1222 of Human ErbB3/ HER3.
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Positive control
- Lysate of SKBR3 cells treated with neuregulin.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.31 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5807 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ErbB3 / HER3 (phospho Y1222) antibody [EPR5807] (ab133445) at 1/1000 dilution
Lane 1 : SKBR3 cell lysate
Lane 2 : Lysate of SKBR3 cells treated with neuregulin.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
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