Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)
![Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)](https://www.abcam.com/ps/products/256/ab256504/Images/ab256504-4-anti-erbb-3-antibody-epr22669-25-immunocytochemistry-skin-mouse.jpg "Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22669-25] to ErbB3 / HER3 - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free
See all ErbB3 / HER3 primary antibodies -
Description
Rabbit monoclonal [EPR22669-25] to ErbB3 / HER3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human bladder cancer and breast cancer tissue. Mouse skin tissue. Flow Cyt: MCF7 cells.
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General notes
Ab256504 is the carrier-free version of ab255607. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256504 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22669-25 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)
Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling ErbB 3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in mouse skin (PMID:26194695) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255607).
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![Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)](https://www.abcam.com/ps/products/256/ab256504/Images/ab256504-3-anti-erbb-3-antibody-epr22669-25-immunocytochemistry-breast-cancer-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ErbB 3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in human breast carcinoma (PMID: 24346286) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255607).
-
![Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)](https://www.abcam.com/ps/products/256/ab256504/Images/ab256504-2-anti-erbb-3-antibody-epr22669-25-immunocytochemistry-bladder-cancer-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling ErbB 3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in human urothelial carcinoma (PMID: 23333248) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255607).
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![Flow Cytometry - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)](https://www.abcam.com/ps/products/256/ab256504/Images/ab256504-1-anti-erbb-3-antibody-epr22669-25-flowcytometry-mcf7-mdamb231-human.jpg)
Flow Cytometry - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MDA-MB-231 (Human breast adenocarcinoma epithelial cell, Left) / MCF7 (Human breast adenocarcinoma epithelial cell, Right) cells labeling ErbB 3 with ab255607 at 1/50 dilution (1μg)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Weak expression control: MDA-MB-231 (PMID: 25018110, 16519802).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255607). -
![Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)](https://www.abcam.com/ps/products/256/ab256504/Images/ab256504-5-benefits-of-recombinant-antibodies.png)
Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)
