Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22669-25] to ErbB3 / HER3 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free
    See all ErbB3 / HER3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22669-25] to ErbB3 / HER3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human bladder cancer and breast cancer tissue. Mouse skin tissue. Flow Cyt: MCF7 cells.

  • General notes

    Ab256504 is the carrier-free version of ab255607. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256504 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)
    Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling ErbB 3 with ab255607 at 1/250 dilution (2.1 μg/ml)  followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in mouse skin (PMID:26194695) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255607).

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)
    Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ErbB 3 with ab255607 at 1/250 dilution (2.1 μg/ml)  followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in human breast carcinoma (PMID: 24346286) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255607).

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)
    Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling ErbB 3 with ab255607 at 1/250 dilution (2.1 μg/ml)  followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in human urothelial carcinoma (PMID: 23333248) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255607).

  • Flow Cytometry - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)
    Flow Cytometry - Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)

    Flow cytometric analysis of 4% paraformaldehyde  fixed 90% methanol  permeabilized MDA-MB-231 (Human breast adenocarcinoma epithelial cell, Left) / MCF7 (Human breast adenocarcinoma epithelial cell, Right) cells labeling ErbB 3 with ab255607 at 1/50 dilution (1μg)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Weak expression control: MDA-MB-231 (PMID: 25018110, 16519802).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255607).
  • Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)
    Anti-ErbB3 / HER3 antibody [EPR22669-25] - BSA and Azide free (ab256504)

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