Anti-c-Fos antibody [EPR883(2)] - BSA and Azide free (ab218578)
![Anti-c-Fos antibody [EPR883(2)] - BSA and Azide free (ab218578)](https://www.abcam.com/ps/products/218/ab218578/Images/ab218578-326485-anti-c-fos-antibody-epr8832-flow-cytometry.jpg "Anti-c-Fos antibody [EPR883(2)] - BSA and Azide free (ab218578)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR883(2)] to c-Fos - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-c-Fos antibody [EPR883(2)] - BSA and Azide free
See all c-Fos primary antibodies -
Description
Rabbit monoclonal [EPR883(2)] to c-Fos - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Lysates of U937 cells treated with TPA and untreated U937 cells, Lysate of HeLa cells treated with TPA.
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General notes
Ab218578 is the carrier-free version of ab134122. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab218578 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR883(2) -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-c-Fos antibody [EPR883(2)] - BSA and Azide free (ab218578)Overlay histogram showing HeLa cells stained with ab134122 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134122, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134122).
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![Anti-c-Fos antibody [EPR883(2)] - BSA and Azide free (ab218578)](https://www.abcam.com/ps/products/218/ab218578/Images/ab218578-2-benefits-of-recombinant-antibodies.png)
Anti-c-Fos antibody [EPR883(2)] - BSA and Azide free (ab218578)
