Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-c-Fos antibody [EPR20769] (ab214672)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20769] to c-Fos
Suitable for: IP, ICC/IF, WB
Reacts with: Mouse, Human

Product name

Anti-c-Fos antibody [EPR20769]
See all c-Fos primary antibodies
Description

Rabbit monoclonal [EPR20769] to c-Fos
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

Recombinant fragment within Human c-Fos aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P01100

Run BLAST with Run BLAST with
Positive control
- WB: RAW264.7, Jurkat (PMA treated) and HeLa (20% FBS 2 hours) whole cell lysates. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20769
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR20769] (ab214672)

Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized serum treated and non-treated HeLa (human cervix adenocarcinoma epithelial cell) cells labeling c-Fos with ab214672 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing weakly nuclear staining on HeLa cells grown in serum free medium for 36 hours. Expression of c-Fos increased in HeLa cells grown in serum free medium for 36 hours followed by addition of 20% FBS for 2 hours.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunoprecipitation - Anti-c-Fos antibody [EPR20769] (ab214672)

c-Fos was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab214672 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214672 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) grown in serum free medium for 36 hours, followed by addition of 20%FBS for 2 hours, whole cell lysate, 10 μg (Input).
Lane 2: ab214672 IP in HeLa grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214672 in HeLa grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours, whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.

The observed lower band is a proteasomal degradation fragment (PMID: 9737957).
Western blot - Anti-c-Fos antibody [EPR20769] (ab214672)

All lanes : Anti-c-Fos antibody [EPR20769] (ab214672) at 1/1000 dilution

Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) grown in serum free medium for 36 hours, whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression of c-Fos is induced by the addition of 20%FBS (PMID: 24386331, PMID: 23300800, PMID: 25695333).
Western blot - Anti-c-Fos antibody [EPR20769] (ab214672)

All lanes : Anti-c-Fos antibody [EPR20769] (ab214672) at 1/5000 dilution

Lane 1 : Untreated Jurkat (human T cell leukemia cell line from peripheral blood) grown in serum free medium overnight, whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) grown in serum free medium overnight, followed by treatment with 200 nM PMA for 4 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes.

Blocking/Dilution buffer: 5% NFDM/TBST.

PMA treatment induces expression of c-Fos, as documented in the literature (PMID: 24386331, PMID: 23300800, PMID: 25695333).
Western blot - Anti-c-Fos antibody [EPR20769] (ab214672)

All lanes : Anti-c-Fos antibody [EPR20769] (ab214672) at 1/5000 dilution

Lane 1 : Untreated RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus grown in serum free medium overnight, whole cell lysate
Lane 2 : RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) grown in serum free medium overnight, followed by treatment with 200 nM PMA for 4 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute.

Blocking/Dilution buffer: 5% NFDM/TBST.

PMA treatment induces expression of c-Fos, as documented in the literature (PMID: 24386331, PMID: 23300800, PMID: 25695333).
Anti-c-Fos antibody [EPR20769] (ab214672)

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