Anti-c-Fos antibody (ab190289)
Key features and details
- Rabbit polyclonal to c-Fos
- Suitable for: IHC-FrFl, ICC, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-c-Fos antibody
See all c-Fos primary antibodies -
Description
Rabbit polyclonal to c-Fos -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P MouseWB MouseHuman
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Immunogen
Recombinant full length protein corresponding to Human c-Fos aa 1 to the C-terminus. expressed in and purified from E. coli.
Database link: P01100 -
Positive control
- WB: HeLa and rat cortical membrane cells.
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Fos antibody (ab190289)All lanes : Anti-c-Fos antibody (ab190289) at 1/2000 dilution
Lane 1 : Protein standard
Lane 2 : HeLa cells grown in FBS free media
Lane 3 : HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours
Lane 4 : Rat cortical neurons
Lane 5 : Rat cortical neurons treated with membrane depolarization buffer for 5 hours
Predicted band size: 41 kDaMouse Monoclonal Antibody to GAPDH, dilution 1/5000, was used as a loading control (red).
Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein.
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Immunohistochemistry - Free Floating - Anti-c-Fos antibody (ab190289)Immunohistochemistry - Free Floating analysis of mouse hippocampus (C) or olfactory bulb sections (D) labeling c-Fos wiht ab190289 at 1/20,000 (red) and mouse mAb to NF-L at 1/5,000 (green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells. -
Immunocytochemistry - Anti-c-Fos antibody (ab190289)Immunocytochemistry analysis of HeLa cells labeling c-Fos with ab190289 at 1/5000 dilution (red) and mouse mAb to tubulin at 1/10,000 dilution (green). The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) – free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells.
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Western blot - Anti-c-Fos antibody (ab190289)All lanes : Anti-c-Fos antibody (ab190289) at 1/2000 dilution
Lane 1 : HeLa cell lysate from cells serum-starved for 36 hours
Lane 2 : HeLa cell lysate from cells serum-starved for 36 hours then stimulated with 20% fetal bovine serum for 2 hours
Predicted band size: 41 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody (ab190289)Immunohistochemistry analysis of mouse brain sections across hippocampus labeling c-Fos with ab190289 at 1/2000 dilution (red). Cells were counterstained with anti Fox3/NeuN antibody (green) and DAPI (blue). Neurons positive for c-Fos and Fox3/NeuN appear to be yellow. Inset shows an enlarged image of c-Fos staining.
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Immunocytochemistry - Anti-c-Fos antibody (ab190289)Immunofluorescent analysis of HeLa cells (serum–starved, 36 hours followed by 20% FBS treatment for 2 hours) labeling c-Fos with ab190289 at 1/2000 dilution (red). Cells were counterstained with chicken polyclonal antibody against vimentin (green) and DAPI (blue).
