Alexa Fluor® 488 Anti-Coilin antibody [IH10] (ab197531)
![Alexa Fluor® 488 Anti-Coilin antibody [IH10] (ab197531)](https://www.abcam.com/ps/products/197/ab197531/Images/ab197531-257022-anti-coilin-antibody-ih10-alexa-fluor-488-flow-cytometry.jpg "Alexa Fluor® 488 Anti-Coilin antibody [IH10] (ab197531)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [IH10] to Coilin
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2b
Overview
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Product name
Alexa Fluor® 488 Anti-Coilin antibody [IH10]
See all Coilin primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [IH10] to Coilin -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Synthetic peptide corresponding to Human Coilin.
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Positive control
- ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
IH10 -
Isotype
IgG2b -
Research areas
Images
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Flow Cytometry - Alexa Fluor® 488 Anti-Coilin antibody [IH10] (ab197531)
Overlay histogram showing HeLa cells stained with ab197531 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197531, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b (monoclonal) Alexa Fluor® 488 (ab171465) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Coilin antibody [IH10] (ab197531)](https://www.abcam.com/ps/products/197/ab197531/Images/ab197531-239479-anti-coilin-antibody-ih10-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Coilin antibody [IH10] (ab197531)ab197531 staining Coilin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197531 at 1/1000 dilution (shown in green) and ab6046 (Rabbit polyclonal to beta Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150088, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), pre-adsorbed, at 1µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
