Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6235(2)] to IKB alpha (phospho S36) - BSA and Azide free
  • Suitable for: Dot blot, Flow Cyt, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free
    See all IKB alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR6235(2)] to IKB alpha (phospho S36) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, Flow Cyt, WBmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa cells treated with FBS and Calyculin A.

  • General notes

    ab240059 is the carrier-free version of ab133462 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240059 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)
    Dot Blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)

    Dot blot analysis of IKB alpha (phospho S36) phospho peptide (Lane 1) and IKB alpha non-phospho peptide (Lane 2) labelling IKB alpha (phospho S36) phospho peptide with ab133462 at a dilution of 1:1000 dilution (0.049μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

    Blocking buffer: 5% NFDM/TBST.
    Dilution buffer: 5% NFDM /TBST .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133462).

  • Flow Cytometry - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)
    Flow Cytometry - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) untreated/treated with 1% FBS for 3 hours then 100 nM Calyculin A for 10 minutes cells labeling IKB alpha (phospho S36) with unpurified ab133462 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised using 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black) (ab172730). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). Untreated control (Green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133462).

  • Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)
    Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)

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