Anti-IKB alpha (phospho Y305) antibody (ab24784)
Key features and details
- Rabbit polyclonal to IKB alpha (phospho Y305)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IKB alpha (phospho Y305) antibody
See all IKB alpha primary antibodies -
Description
Rabbit polyclonal to IKB alpha (phospho Y305) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human IKB alpha (phospho Y305) conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide conjugated to KLH corresponding to amino acid residues around tyrosine 305 of human IKB alpha. This peptide sequence has low homology to other IKB proteins.
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Positive control
- A431 and Jurkat cells treated with pervanadate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho IKB alpha (Tyr-305) peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IKB alpha (phospho Y305) antibody (ab24784)Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 minutes (30 ug/lane). Blots were probed with anti-IkappaBalpha (lane 1), anti-phospho-IkappaBalpha?(Tyr-42) (ab24783; lanes 2-5), or anti-phospho-IkappaBalpha (Tyr-305) (ab24784; lanes 6-9). Both anti-phospho-IkappaBalpha (Tyr-42) and anti-phospho-IkappaBalpha (Tyr-305) were used in the presence of no blocking peptide (lane 2 & 6), phospho-IkappaBalpha (Tyr-42) peptide (lane 3 & 8), phospho-IkappaBalpha (Tyr-305) peptide (lane 4 & 7), or BSA conjugated to phospho-tyrosine (lane 5 & 9). Peptides and BSA-pTyr were used at 1 ug/ml.
