Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 02, 2021

Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3148] to IKB alpha (phospho S32)
Suitable for: Dot blot, WB, IP
Reacts with: Human

Product name

Anti-IKB alpha (phospho S32) antibody [EPR3148]
See all IKB alpha primary antibodies
Description

Rabbit monoclonal [EPR3148] to IKB alpha (phospho S32)
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human IKB alpha aa 1-100 (phospho S32). The exact sequence is proprietary.
Database link: P25963
Positive control
- WB: TNF-a treated HeLa and TNF-a treated MCF7 whole cell lysates; IP: TNF-a treated HeLa whole cell lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3148
Isotype

IgG
Research areas

Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)

All lanes : Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700) at 1/1000 dilution (Purified)

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) treated with 20 ng/mL TNF-alpha for 8 hours whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking/Diluting buffer: 5% NFDM/TBST
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)

All lanes : Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20 ng/mL TNF-alpha for 5 minutes whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking/Diluting buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)

Purified ab92700 at 1:20 dilution (1μg) immunoprecipitating IKB alpha in HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.

Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate 10μg.
Lane 2 (+): ab92700 + HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92700 in HeLa treated with 20ng/mL TNF-alpha for 60 minutes whole cell lysate.

Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 36 kDa
Western blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)

All lanes : Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700) at 1/500 dilution (unpurified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with TNF-a at 20 ng/mL for 5 minutes. Whole cell lysates
Lane 3 : HeLa treated with TNF-a at 20 ng/mL for 5 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 3 minutes

Blocking/Diluting buffer and concentration 5% NFDM/TBST
Dot Blot - Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)

Dot blot analysis of IKB alpha (phospho S32) phospho peptide (Lane 1) and IKB alpha non-phospho peptide (Lane 2) labeling IKB alpha (phospho S32) with unpurified ab92700 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/10000 was used as the secondary antibody.

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-IKB alpha (phospho S32) antibody [EPR3148] (ab92700)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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