Human SQSTM1 (p62) knockout HCT116 cell lysate (ab257052)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263488 - Human SQSTM1 knockout HCT116 cell lysate 1 x 100µg ab255555 - Human wild-type HCT116 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X D5S818: 10, 11 D13S317: 10, 12 D7S820: 11, 12 D16S539: 11, 13 vWA: 17, 22 TH01: 8,9 TPOX: 8, 9 CSF1PO: 7, 10
Images
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Western blot - Human SQSTM1 (p62) knockout HCT116 cell lysate (ab257052)
Lane 1:Wild-type HCT116 cell lysate (20 ug)
Lane 2:SQSTM1 knockout HCT116 cell lysate (20 ug)
Lane 3:HepG2 cell lysate (20 ug)
Lane 4:HeLa cell lysate (20 ug)ab109012 was shown to specifically react with SQSTM1 / p62 in wild-type HCT116 cells. The band observed in knockout cell line ab266871 (knockout cell lysate ab257052) lane below 55 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and SQSTM1 / p62 knockout samples were subjected to SDS-PAGE. ab109012 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human SQSTM1 (p62) knockout HCT116 cell lysate (ab257052)Lane 1:Wild-type HCT116 cell lysate (20 ug)
Lane 2:SQSTM1 knockout HCT116 cell lysate (20 ug)
Lane 3:HepG2 cell lysate (20 ug)
Lane 4:HeLa cell lysate (20 ug)ab207305 was shown to specifically react with SQSTM1 / p62 in wild-type HCT116 cells. The band observed in knockout cell line ab266871 (knockout cell lysate ab257052) lane below 55 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and SQSTM1 / p62 knockout samples were subjected to SDS-PAGE. ab207305 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human SQSTM1 knockout HCT116 cell lysate (ab257052)Homozygous: 49 bp deletion in exon4
