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Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway

Anti-IKB alpha antibody (ab97783)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-IKB alpha antibody (ab97783)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to IKB alpha
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-IKB alpha antibody
    See all IKB alpha primary antibodies
  • Description

    Rabbit polyclonal to IKB alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human IKB alpha (internal sequence). a sequence within the center region
    Database link: P25963

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • NFkB pathway
    • Immunology
    • Innate Immunity
    • TLR Signaling

Images

  • Western blot - Anti-IKB alpha antibody (ab97783)
    Western blot - Anti-IKB alpha antibody (ab97783)
    All lanes : Anti-IKB alpha antibody (ab97783) at 1/5000 dilution

    Lane 1 : BCL-1 whole cell lysate
    Lane 2 : C2C12 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10 % SDS-PAGE

  • Western blot - Anti-IKB alpha antibody (ab97783)
    Western blot - Anti-IKB alpha antibody (ab97783)
    Anti-IKB alpha antibody (ab97783) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 36 kDa



    10% SDS-PAGE.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody (ab97783)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody (ab97783)

    ab97783 at 1/500 dilution detects IKB alpha protein at nucleus on U251 xenograft by immunohistochemical analysis.
    Sample: Paraffin-embedded U251 xenograft.

  • Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody (ab97783)
    Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody (ab97783)
    ab97783 at 1/200 dilution staining IKB alpha in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-IKB alpha antibody (ab97783)

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    HRP Anti-IKB alpha antibody [E130] (ab202646)

    Applications: IHC-P, WB

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    Applications: Dot, IP, WB

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    Anti-IKB alpha antibody [E130] (ab32518)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Anti-IKB alpha antibody [EP697] (ab76429)

    Applications: ICC/IF, IHC-P, WB

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    Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462)

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    Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)

    Applications: Dot, Flow Cyt, WB

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    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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