Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 02, 2021

Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6235(2)] to IKB alpha (phospho S36)
Suitable for: Flow Cyt, WB, Dot blot
Reacts with: Human

Product name

Anti-IKB alpha (phospho S36) antibody [EPR6235(2)]
See all IKB alpha primary antibodies
Description

Rabbit monoclonal [EPR6235(2)] to IKB alpha (phospho S36)
Host species

Rabbit
Specificity

This antibody detects IKB alpha only when phosphorylated at Serine 36. Stimulation may be required to allow detection of the phosphorylated protein due to low levels of endogenous expression. Please see images below for recommended treatment conditions and positive controls.
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human IKB alpha.
Positive control
- HeLa cells treated with FBS and Calyculin A.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6235(2)
Isotype

IgG
Research areas

Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462)

All lanes : Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) at 1/1000 dilution

Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2 : THP-1 treated with 100ng/ml IFN-gamma for 24hours whole cell lysate
Lane 3 : THP-1 treated with 100ng/ml IFN-gamma for 24hours whole cell lysate. Then the membrane was incubated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 35 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Dot Blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462)

Dot blot analysis of IKB alpha (phospho S36) phospho peptide (Lane 1) and IKB alpha non-phospho peptide (Lane 2) labelling IKB alpha (phospho S36) phospho peptide with ab133462 at a dilution of 1/1000 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462)

All lanes : Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with FBS and Calyculin A

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 35 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) untreated/treated with 1% FBS for 3 hours then 100 nM Calyculin A for 10 minutes cells labeling IKB alpha (phospho S36) with unpurified ab133462 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised using 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black) (ab172730). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). Untreated control (Green).
Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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