Anti-IKB alpha (phospho Y42) antibody (ab24783)
Key features and details
- Rabbit polyclonal to IKB alpha (phospho Y42)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IKB alpha (phospho Y42) antibody
See all IKB alpha primary antibodies -
Description
Rabbit polyclonal to IKB alpha (phospho Y42) -
Host species
Rabbit -
Specificity
There may be some homology to unrelated proteins with a conserved tyrosine phosphorylation motif. It is recommended that this antibody be used after immunoprecipitation with an IKB alpha specific antibody. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to amino acid residues around tyrosine 42 of human IKB alpha, conjugated to KLH
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General notes
We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.Useful in detection of phosphorylated IKB alpha (Tyr-42).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Cross absorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IKB alpha (Tyr-42) peptide, without carrier. -
Primary antibody notes
Useful in detection of phosphorylated IKB alpha (Tyr-42). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IKB alpha (phospho Y42) antibody (ab24783)Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 minutes (30 ug/lane). Blots were probed with anti-IkappaBalpha (lane 1), anti-phospho-IkappaBalpha?(Tyr-42) (ab24783; lanes 2-5), or anti-phospho-IkappaBalpha (Tyr-305) (ab24784; lanes 6-9). Both anti-phospho-IkappaBalpha (Tyr-42) and anti-phospho-IkappaBalpha (Tyr-305) were used in the presence of no blocking peptide (lane 2 & 6), phospho-IkappaBalpha (Tyr-42) peptide (lane 3 & 8), phospho-IkappaBalpha (Tyr-305) peptide (lane 4 & 7), or BSA conjugated to phospho-tyrosine (lane 5 & 9). Peptides and BSA-pTyr were used at 1 ug/ml.
