Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Wednesday March 17, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4236] to GST
Suitable for: WB, ICC/IF, IP
Reacts with: Species independent

Product name

Anti-GST antibody [EPR4236]
See all GST primary antibodies
Description

Rabbit monoclonal [EPR4236] to GST
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivity

Reacts with: Species independent
Immunogen

Synthetic peptide (GST-tag) within GST aa 300-450. The exact sequence is proprietary. GST tag was fused to ATG16L1 to produce a recombinant protein.
Database link: Q676U5
Positive control
- GST fusion proteins; Alpha-actinin-4-GST, AKT1/PKBa-GST or CDK7-GST.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4236
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (ab111947)

Immunofluorescence staining of 293T cells transfected with GST, with ab111947 at a working dilution of 1/100 and counter-stained with DAPI. Tubulin was also stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor^® 594 goat anti-mouse at a dilution of 1/500 (ab150120). The secondary antibody was Alexa Fluor^® 488 goat anti rabbit (ab150077), used at a dilution of 1/500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X-100. The negative controls are shown in the bottom hand panels - for the first negative control, anti-GST antibody (ab111947) was used at a dilution of 1/100 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000 and for the second negative control, mouse anti-tubulin (ab7291) and anti-rabbit secondary antibody (ab150077) were used.
Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)

GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: ab111947, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: ab111947, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Lane 3: Primary: ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysate
All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
Blocking and diluting buffer: 5% NFDM/TBST

Lanes 1-2 : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
Lane 3 : Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Lane 1 : Input - 293 transfected with GST tagged protein vector cell lysate at 10 µg
Lane 2 : (+) 293T transfected with GST tagged protein vector cell lysate
Lane 3 : (-) 293T transfected with GST tagged protein vector cell lysate

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution (VeriBlot)

Additional bands at: 41 kDa (possible tagged protein)
Western blot - Anti-GST antibody [EPR4236] (ab111947)

Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution + GST recombinant protein at 0.01 µg

Predicted band size: 26 kDa
Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)

(Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Secondary: ab131366, Veriblot, 1/10000 dilution
Lane 3: Primary: ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysate
All lanes: Secondary: ab131366, Veriblot, 1/10000 dilution
Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-GST antibody [EPR4236] (ab111947)

All lanes : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution

Lane 1 : Alpha-actinin-4-GST fusion protein
Lane 2 : AKT1/PKBa-GST fusion protein
Lane 3 : CDK7-GST fusion protein

Lysates/proteins at 0.01 µg per lane.

Predicted band size: 26 kDa
Anti-GST antibody [EPR4236] (ab111947)

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