Recombinant human CD27 protein (Fc Chimera) (ab180048)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.4
Constituents: 0.6% Tris, 0.75% Glycine, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 1 mg/ml.
Images
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SDS-PAGE - Recombinant human CD27 protein (Fc Chimera) (ab180048)Human CD27, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human CD27 protein (Fc Chimera) (ab180048)Immobilized Human CD27, Fc Tag at 2 µg/mL (100 µL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag with a linear range of 1-31 ng/mL. -
SDS-PAGE - Recombinant human CD27 protein (Fc Chimera) (ab180048)The purity of ab180048 was determined by reduced SDS-PAGE and staining overnight with Coomassie Blue. DTT-reduced protein migrates as 45-65 kDa due to glycosylation.
