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Immunology Adaptive Immunity T Cells CD

Anti-CD22 antibody [2H1C4] (ab181771)

Price and availability

274 732 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-CD22 antibody [2H1C4] (ab181771)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [2H1C4] to CD22
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD22 antibody [2H1C4]
    See all CD22 primary antibodies
  • Description

    Mouse monoclonal [2H1C4] to CD22
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human CD22 aa 621-725. expressed in E. Coli.
    Sequence:

    NPPVSHYTWFDWNNQSLPYHSQKLRLEPVKVQHSGAYWCQGTNSVGKGRS PLSTLTVYYSPETIGRRVAVGLGSCLAILILAICGLKLQRRWKRTQSQQG LQENS


    Database link: P20273
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human CD22 recombinant protein; HEK293 cell lysate, transfected with CD22 (amino acids 622-725); Mouse L1210, HeLa, HEK293, Jurkat, OCM-1, A432 and mouse NIH 3T3 cell lysates; HeLa cells; Human cervical cancer and cerebellum tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2H1C4
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage

Images

  • Flow Cytometry - Anti-CD22 antibody [2H1C4] (ab181771)
    Flow Cytometry - Anti-CD22 antibody [2H1C4] (ab181771)

    Flow cytometric analysis of HeLa cells labeling CD22 with ab181771 at 1/200 dilution (green); negative control (red).

  • Western blot - Anti-CD22 antibody [2H1C4] (ab181771)
    Western blot - Anti-CD22 antibody [2H1C4] (ab181771)
    Anti-CD22 antibody [2H1C4] (ab181771) at 1/500 dilution + Human CD22 recombinant protein

    Predicted band size: 95 kDa

  • Western blot - Anti-CD22 antibody [2H1C4] (ab181771)
    Western blot - Anti-CD22 antibody [2H1C4] (ab181771)
    All lanes : Anti-CD22 antibody [2H1C4] (ab181771) at 1/500 dilution

    Lane 1 : HEK293 cell lysate, non-transfected
    Lane 2 : HEK293 cell lysate, transfected with CD22 (amino acids 622-725)-hIgGFc

    Predicted band size: 95 kDa

  • Western blot - Anti-CD22 antibody [2H1C4] (ab181771)
    Western blot - Anti-CD22 antibody [2H1C4] (ab181771)
    All lanes : Anti-CD22 antibody [2H1C4] (ab181771) at 1/500 dilution

    Lane 1 : mouse L1210 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HEK293 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : OCM-1 cell lysate
    Lane 6 : A431 cell lysate
    Lane 7 : mouse NIH 3T3 cell lysate

    Predicted band size: 95 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [2H1C4] (ab181771)
    Immunocytochemistry/ Immunofluorescence - Anti-CD22 antibody [2H1C4] (ab181771)

    Immunofluorescent analysis of HeLa cells, labeling CD22 with ab181771 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [2H1C4] (ab181771)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [2H1C4] (ab181771)

    Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling CD22 with ab181771 at 1/200 dilution followed by DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [2H1C4] (ab181771)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD22 antibody [2H1C4] (ab181771)

    Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling CD22 with ab181771 at 1/200 dilution followed by DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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