Anti-eIF4B (phospho S422) antibody (ab59300)
Key features and details
- Rabbit polyclonal to eIF4B (phospho S422)
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4B (phospho S422) antibody
See all eIF4B primary antibodies -
Description
Rabbit polyclonal to eIF4B (phospho S422) -
Host species
Rabbit -
Specificity
Detects endogenous levels of eIF4B only when phosphorylated at serine 422. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of serine 422 (T-G-SP-E-S).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4B (phospho S422) antibody (ab59300)All lanes : Anti-eIF4B (phospho S422) antibody (ab59300) at 1/500 dilution
Lane 1 : extracts from NIH/3T3cells
Lane 2 : extracts from NIH/3T3cells with immunizing peptide
Predicted band size: 69 kDa
Observed band size: 69 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B (phospho S422) antibody (ab59300)Ab59300 at 1/50 dilution staining human colon carcinoma without (left) and with (right) immunizing peptide; paraffin embedded. -
Immunocytochemistry/ Immunofluorescence - Anti-eIF4B (phospho S422) antibody (ab59300)ICC/IF image of ab59300 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59300, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
