Anti-eIF4B (phospho S406) antibody [EPR7683] (ab134138)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7683] to eIF4B (phospho S406)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-eIF4B (phospho S406) antibody [EPR7683]
See all eIF4B primary antibodies -
Description
Rabbit monoclonal [EPR7683] to eIF4B (phospho S406) -
Host species
Rabbit -
Specificity
ab134138 only detects eIF4B phosphorylated at Serine 406. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Specific peptide corresponding to residues surrounding phosphorylated Serine 406 of Human eIF4B.(Uniprot ID: P23588)
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Positive control
- HeLa cell lysate and Human colon tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.35 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7683 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-eIF4B (phospho S406) antibody [EPR7683] (ab134138) at 1/10000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Ani-rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa
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Immunohistochemical analysis of Paraffin-embedded Human colon tissue labelling eIF4B phosphorylated at S406 with ab134138 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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