Anti-TRAP1 antibody [EPR5381] (ab109323)
![Anti-TRAP1 antibody [EPR5381] (ab109323)](https://www.abcam.com/ps/products/109/ab109323/Images/ab109323-122103-anti-trap1-antibody-epr5381-western-blot.jpg "Anti-TRAP1 antibody [EPR5381] (ab109323)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5381] to TRAP1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-TRAP1 antibody [EPR5381]
See all TRAP1 primary antibodies -
Description
Rabbit monoclonal [EPR5381] to TRAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562, HL60, 293T and HeLa cell lysates IHC-P: Human kidney and liver tissues
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5381 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRAP1 antibody [EPR5381] (ab109323)All lanes : Anti-TRAP1 antibody [EPR5381] (ab109323) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 80 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (ab109323)](https://www.abcam.com/ps/products/109/ab109323/Images/ab109323-122102-anti-trap1-antibody-epr5381-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (ab109323)ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (ab109323)](https://www.abcam.com/ps/products/109/ab109323/Images/ab109323-122101-anti-trap1-antibody-epr5381-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAP1 antibody [EPR5381] (ab109323)ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-TRAP1 antibody [EPR5381] (ab109323)](https://www.abcam.com/ps/products/109/ab109323/Images/ab109323-4-benefits-of-recombinant-antibodies.png)
Anti-TRAP1 antibody [EPR5381] (ab109323)
