Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERp57 antibody [CL2444] (ab242367)

Key features and details

  • Mouse monoclonal [CL2444] to ERp57
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ERp57 antibody [CL2444]
    See all ERp57 primary antibodies

  • Description

    Mouse monoclonal [CL2444] to ERp57

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human ERp57 aa 100-250.
    Database link: P30101

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    Binds to an epitope located within the peptide sequence KKFISDKDASIVGFF as determined by overlapping synthetic peptides.

  • Positive control

    • WB: U-251 MG cell lysate. IHC-P: Human thyroid gland, cerebral cortex and endometrium tissue. ICC/IF: HeLa, A431, MCF7, U-2 OS and U-251 MG cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    CL2444

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)

    Immunofluorescence/immunocytochemical analysis of U-251 MG (Human brain glioma cell line) cells labeling ERp57 (Green) using ab242367 at 4 µg/mL. The nuclear counterstain is DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)

    Immunofluorescence/immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling ERp57 (Green) using ab242367 at 4 µg/mL. The nuclear counterstain is DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)

    Immunofluorescence/immunocytochemical analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling ERp57 (Green) using ab242367 at 4 µg/mL. The nuclear counterstain is DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)

    Immunofluorescence/immunocytochemical analysis of A431 (human epidermoid carcinoma cell line) cells labeling ERp57 (Green) using ab242367 at 4 µg/mL. The nuclear counterstain is DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)

    Immunofluorescence/immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ERp57 (Green) using ab242367 at 4 µg/mL. The nuclear counterstain is DAPI (Blue).

  • Western blot - Anti-ERp57 antibody [CL2444] (ab242367)
    Western blot - Anti-ERp57 antibody [CL2444] (ab242367)
    All lanes : Anti-ERp57 antibody [CL2444] (ab242367) at 1 µg/ml

    Lane 1 : U-251 MG (Human brain glioma cell line) cells transfected with a control siRNA
    Lane 2 : U-251 MG cells transfected with a target-specific siRNA probe
    Lane 3 : U-251 MG cells transfected with a second target-specific siRNA probe

    Predicted band size: 57 kDa

  • Western blot - Anti-ERp57 antibody [CL2444] (ab242367)
    Western blot - Anti-ERp57 antibody [CL2444] (ab242367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)

    Paraffin-embedded human endometrium tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)

    Paraffin-embedded human cerebral cortex tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)

    Paraffin-embedded human thyroid gland tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)

    Negative control:

    Paraffin-embedded human skeletal muscle tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

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