Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [CL2444] to ERp57
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-ERp57 antibody [CL2444]
See all ERp57 primary antibodies
Description

Mouse monoclonal [CL2444] to ERp57
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human ERp57 aa 100-250.
Database link: P30101

Run BLAST with Run BLAST with
Epitope

Binds to an epitope located within the peptide sequence KKFISDKDASIVGFF as determined by overlapping synthetic peptides.
Positive control
- WB: U-251 MG cell lysate. IHC-P: Human thyroid gland, cerebral cortex and endometrium tissue. ICC/IF: HeLa, A431, MCF7, U-2 OS and U-251 MG cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

CL2444
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)

Immunofluorescence/immunocytochemical analysis of U-251 MG (Human brain glioma cell line) cells labeling ERp57 (Green) using ab242367 at 4 µg/mL. The nuclear counterstain is DAPI (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)

Immunofluorescence/immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling ERp57 (Green) using ab242367 at 4 µg/mL. The nuclear counterstain is DAPI (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)

Immunofluorescence/immunocytochemical analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling ERp57 (Green) using ab242367 at 4 µg/mL. The nuclear counterstain is DAPI (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)

Immunofluorescence/immunocytochemical analysis of A431 (human epidermoid carcinoma cell line) cells labeling ERp57 (Green) using ab242367 at 4 µg/mL. The nuclear counterstain is DAPI (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (ab242367)

Immunofluorescence/immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ERp57 (Green) using ab242367 at 4 µg/mL. The nuclear counterstain is DAPI (Blue).
Western blot - Anti-ERp57 antibody [CL2444] (ab242367)

All lanes : Anti-ERp57 antibody [CL2444] (ab242367) at 1 µg/ml

Lane 1 : U-251 MG (Human brain glioma cell line) cells transfected with a control siRNA
Lane 2 : U-251 MG cells transfected with a target-specific siRNA probe
Lane 3 : U-251 MG cells transfected with a second target-specific siRNA probe

Predicted band size: 57 kDa
Western blot - Anti-ERp57 antibody [CL2444] (ab242367)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)

Paraffin-embedded human endometrium tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)

Paraffin-embedded human cerebral cortex tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)

Paraffin-embedded human thyroid gland tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (ab242367)

Negative control:

Paraffin-embedded human skeletal muscle tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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