Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ERp57 antibody [EPR10679(B)] (ab154197)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10679(B)] to ERp57
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-ERp57 antibody [EPR10679(B)]
See all ERp57 primary antibodies
Description

Rabbit monoclonal [EPR10679(B)] to ERp57
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide, corresponding to residues in Human ERp57 (UniProt: P30101).
Positive control
- HeLa, HepG2, Daudi and 293T cell lysates; Human pancreas and stomach tissues.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10679(B)
Isotype

IgG
Research areas

Western blot - Anti-ERp57 antibody [EPR10679(B)] (ab154197)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ERp57 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Hek293 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab154197 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab154197 was shown to specifically react with ERp57 in wild-type HAP1 cells as signal was lost in ERp57 knockout cells. Wild-type and ERp57 knockout samples were subjected to SDS-PAGE. ab154197 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] (ab154197)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ERp57 with ab154197 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-ERp57 antibody [EPR10679(B)] (ab154197)

All lanes : Anti-ERp57 antibody [EPR10679(B)] (ab154197) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Daudi cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] (ab154197)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling ERp57 with ab154197 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] (ab154197)

Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154197 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] (ab154197)

Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154197 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] (ab154197)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154197 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] (ab154197)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154197 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-ERp57 antibody [EPR10679(B)] (ab154197)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com