Anti-RUNX1T1/ETO/CDR antibody - ChIP Grade (ab195329)
Key features and details
- Rabbit polyclonal to RUNX1T1/ETO/CDR - ChIP Grade
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RUNX1T1/ETO/CDR antibody - ChIP Grade
See all RUNX1T1/ETO/CDR primary antibodies -
Description
Rabbit polyclonal to RUNX1T1/ETO/CDR - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP Human
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Immunogen
This product was produced with the following immunogens:
Synthetic peptide within Human RUNX1T1/ETO/CDR aa 50-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q06455
Synthetic peptide within Human RUNX1T1/ETO/CDR aa 250-300 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q06455 -
Positive control
- Chromatin from SKNO-1 cells
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General notes
This product was previously labelled as RUNX1T1 / ETO
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-RUNX1T1/ETO/CDR antibody - ChIP Grade (ab195329)
ChIP assays were performed using 1.25 million SKNO-1 cells and 4 μL of ab195329. The IP’d DNA of 6 ChIP’s was pooled and analysed. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Image shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
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ChIP - Anti-RUNX1T1/ETO/CDR antibody - ChIP Grade (ab195329)ChIP assays were performed using SKNO-1 cells, ab195329 and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μL of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Image shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.
