Rabbit Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150147)
Key features and details
- Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (Alexa Fluor® 647), pre-adsorbed
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-Fr, ELISA, ICC/IF, Flow Cyt, IHC-P
Overview
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Product name
Rabbit Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed
See all IgG secondary antibodies -
Description
Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (Alexa Fluor® 647), pre-adsorbed -
Host species
Rabbit -
Target species
Goat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig and rat IgG was detected.
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Tested applications
Suitable for: IHC-Fr, ELISA, ICC/IF, Flow Cyt, IHC-Pmore details -
Minimal
cross-reactivity
Chicken, Horse, Human, Mouse, Pig, Rat more details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150147)ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150147 Alexa Fluor® 647 rabbit anti-goat IgG (H+L) (red) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150147)HeLa cells showing negative staining by ICC/IF using only tertiary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. ab150147 Alexa Fluor® 647 rabbit anti-goat IgG (H+L) (red) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150147)