Anti-ERp57 antibody (ab228789)
Key features and details
- Rabbit polyclonal to ERp57
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ERp57 antibody
See all ERp57 primary antibodies -
Description
Rabbit polyclonal to ERp57 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey
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Immunogen
Recombinant fragment within Human ERp57 (internal sequence). The exact sequence is proprietary.
Database link: P30101 -
Positive control
- WB: HepG2, HCT 116, Neuro-2a, GL261 and C8D30 whole cell lysates; HEK-293T, A431, HeLa, PC-12 and Rat2 whole cell extracts. IHC-P: U87 xenograft tissue; Mouse testis tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERp57 antibody (ab228789)All lanes : Anti-ERp57 antibody (ab228789) at 1/500 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab228789)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ERp57 (green) using ab228789 at 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). alpha Tubulin, a cytoskeleton marker, was stained by an anti-alpha Tubulin antibody at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody (ab228789)Paraffin-embedded mouse testis tissue stained for ERp57 using ab228789 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody (ab228789)Paraffin-embedded U87 (human) xenograft tissue stained for ERp57 using ab228789 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-ERp57 antibody (ab228789)All lanes : Anti-ERp57 antibody (ab228789) at 1/10000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-ERp57 antibody (ab228789)All lanes : Anti-ERp57 antibody (ab228789) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-ERp57 antibody (ab228789)All lanes : Anti-ERp57 antibody (ab228789) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa7.5% SDS-PAGE gel.
