Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERp57 antibody [EPR10678(B)] (ab154191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10678(B)] to ERp57
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ERp57 antibody [EPR10678(B)]
    See all ERp57 primary antibodies

  • Description

    Rabbit monoclonal [EPR10678(B)] to ERp57

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human ERp57 (P30101)

  • Positive control

    • HepG2 cell lysate, 293T cell lysate, HeLa cell lysate, paraffin-embedded Human pancreas tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10678(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
    Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: ERp57 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: Hek293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab154191 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab154191 was shown to specifically react with ERp57 in wild-type HAP1 cells as signal was lost in ERp57 knockout cells. Wild-type and ERp57 knockout samples were subjected to SDS-PAGE. ab154191 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

    Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154191 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
    Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
    All lanes : Anti-ERp57 antibody [EPR10678(B)] (ab154191) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ERp57 with ab154191 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154191 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

    Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue using ab154191 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

    Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154191 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

    Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154191 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-ERp57 antibody [EPR10678(B)] (ab154191)
    Anti-ERp57 antibody [EPR10678(B)] (ab154191)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ERp57 antibody [EPR10678(B)] (ab154191)

© 2021 Astana Biomed Group LLP. All rights reserved.